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Purine Nucleoside Phosphorylase/PNP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19603

Novus Biologicals, part of Bio-Techne
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NBP2-19603

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Rhesus Macaque (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Purine Nucleoside Phosphorylase/PNP. The exact sequence is proprietary.

Reactivity Notes

Bovine (88%), Mouse (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Purine Nucleoside Phosphorylase/PNP Antibody

Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-19603]

Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-19603]

Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-19603] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-19603]

Immunocytochemistry/ Immunofluorescence: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-19603]

Immunocytochemistry/Immunofluorescence: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-19603] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Applications for Purine Nucleoside Phosphorylase/PNP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Purine Nucleoside Phosphorylase/PNP

This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq]

Alternate Names

Inosine phosphorylase, PUNP

Gene Symbol

PNP

UniProt

Additional Purine Nucleoside Phosphorylase/PNP Products

Product Documents for Purine Nucleoside Phosphorylase/PNP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Purine Nucleoside Phosphorylase/PNP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.