Purine Nucleoside Phosphorylase/PNP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99935
![Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-99935]](https://resources.bio-techne.com/images/products/Purine-Nucleoside-Phosphorylase-PNP-Antibody-Western-Blot-NBP2-99935-img0004.jpg "Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-99935]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Purine Nucleoside Phosphorylase/PNP Antibody
Immunogen
Produced in rabbits immunized with purified, recombinant Human Purine Nucleoside Phosphorylase/PNP (Accession#: P00491; Met1-Ser289)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Purine Nucleoside Phosphorylase/PNP Antibody
Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-99935]
Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-99935] - Anti-Purine Nucleoside Phosphorylase/PNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDa![Immunoprecipitation: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-99935]](https://resources.bio-techne.com/images/products/Purine-Nucleoside-Phosphorylase-PNP-Antibody-Immunoprecipitation-NBP2-99935-img0003.jpg "Immunoprecipitation: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-99935]")
Immunoprecipitation: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-99935]
Immunoprecipitation: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-99935] - Purine Nucleoside Phosphorylase/PNP was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg k562 Whole Cell Lysate 2 ul anti-Purine Nucleoside Phosphorylase/PNP rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Purine Nucleoside Phosphorylase/PNP rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDaApplications for Purine Nucleoside Phosphorylase/PNP Antibody
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Purine Nucleoside Phosphorylase/PNP
Alternate Names
Inosine phosphorylase, PUNP
Gene Symbol
PNP
Additional Purine Nucleoside Phosphorylase/PNP Products
Product Documents for Purine Nucleoside Phosphorylase/PNP Antibody
Product Specific Notices for Purine Nucleoside Phosphorylase/PNP Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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