Puromycin-sensitive aminopeptidase/NPEPPS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93421

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Puromycin-sensitive aminopeptidase/NPEPPS Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KRDAESIHQYLLQRK corresponding to internal region according to NP_006301.2.

Epitope

C-KRDAESIHQYLLQRK

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Puromycin-sensitive aminopeptidase/NPEPPS Antibody

Western Blot: Puromycin-sensitive aminopeptidase/NPEPPS Antibody [NB100-93421]

Western Blot: Puromycin-sensitive aminopeptidase/NPEPPS Antibody [NB100-93421]

Western Blot: NPEPPS Antibody [NB100-93421] - (0.3ug/ml) Analysis of Human Brain (Cerebellum) 35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Puromycin-sensitive aminopeptidase/NPEPPS Antibody [NB100-93421]

Western Blot: Puromycin-sensitive aminopeptidase/NPEPPS Antibody [NB100-93421]

Western Blot: NPEPPS Antibody [NB100-93421] - Analysis of NPEPPS in Human Brain (Cerebral Cortex) lysate (35ug protein in RIPA buffer) at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Puromycin-sensitive aminopeptidase/NPEPPS Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000

Western Blot

0.2 - 0.5 ug/ml
Application Notes
WB: Approx. 110 kDa band observed in human brain (cerebral cortex) lysates (calculated MW of 98.5 kDa band according to NP_006301.2).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Puromycin-sensitive aminopeptidase/NPEPPS

This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq]

Alternate Names

AAP-S, MP100, NPEPPS

Entrez Gene IDs

9520 (Human); 19155 (Mouse); 50558 (Rat)

Gene Symbol

NPEPPS

UniProt

NP_006301.2 (Human)

Additional Puromycin-sensitive aminopeptidase/NPEPPS Products

Product Documents for Puromycin-sensitive aminopeptidase/NPEPPS Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Puromycin-sensitive aminopeptidase/NPEPPS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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