PUS1 Antibody (3A3)

Novus Biologicals, part of Bio-Techne | Catalog # H00080324-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3A3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PUS1 Antibodies »

Product Specifications

Immunogen

PUS1 (AAH02901, 1 a.a. ~ 399 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGNAEPPPAGAACPQDRRSCSGRAGGDRVWEDGEHPAKKLKSGGDEERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFAHKDRDVQDETYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTEKVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNFSATALTAGGTGAKVPSPLEGSEGDGDTD

Specificity

Reacts with pseudouridylate synthase 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PUS1 Antibody (3A3)

Western Blot: PUS1 Antibody (3A3) [H00080324-M01]

Western Blot: PUS1 Antibody (3A3) [H00080324-M01]

Western Blot: PUS1 Antibody (3A3) [H00080324-M01] - Analysis of PUS1 expression in transfected 293T cell line by PUS1 monoclonal antibody (M01), clone 3A3.Lane 1: PUS1 transfected lysate(44.4 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PUS1 Antibody (3A3) [H00080324-M01]

Immunocytochemistry/ Immunofluorescence: PUS1 Antibody (3A3) [H00080324-M01]

Immunocytochemistry/Immunofluorescence: PUS1 Antibody (3A3) [H00080324-M01] - Analysis of monoclonal antibody to PUS1 on HeLa cell . Antibody concentration 10 ug/ml.
Immunoprecipitation: PUS1 Antibody (3A3) [H00080324-M01]

Immunoprecipitation: PUS1 Antibody (3A3) [H00080324-M01]

Immunoprecipitation: PUS1 Antibody (3A3) [H00080324-M01] - Analysis of PUS1 transfected lysate using anti-PUS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PUS1 MaxPab rabbit polyclonal antibody.

Applications for PUS1 Antibody (3A3)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PUS1

PUS1 converts uridine into pseudouridine after the nucleotide has been incorporated into RNA. Pseudouridine may have a functional role in tRNAs and may assist in the peptidyl transfer reaction of rRNAs (Chen and Patton, 1999 [PubMed 10094309]).[supplied by OMIM]

Alternate Names

EC 5.4.99.12, MGC11268, mitochondrial tRNA pseudouridine synthase A, MLASA1, pseudouridylate synthase 1, tRNA pseudouridine synthase A, mitochondrial, tRNA pseudouridylate synthase I, tRNA uridine isomerase I, tRNA-uridine isomerase I

Entrez Gene IDs

80324 (Human)

Gene Symbol

PUS1

UniProt

AAH02901 (Human)

Additional PUS1 Products

Product Documents for PUS1 Antibody (3A3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PUS1 Antibody (3A3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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