PUS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-86983

Novus Biologicals, part of Bio-Techne
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NB100-86983
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all PUS1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 377 and 427 of human pseudouridylate synthase 1 using the numbering given in entry NP_079491.2 (GeneID 80324).

Specificity

This antibody detects both isoforms of PUS1: Isoform 1 (47.5 kDa) and Isoform 2 (44.4kDa).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PUS1 Antibody

Western Blot: PUS1 Antibody [NB100-86983]

Western Blot: PUS1 Antibody [NB100-86983]

Western Blot: PUS1 Antibody [NB100-86983] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-86983 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.
Immunohistochemistry: PUS1 Antibody [NB100-86983]

Immunohistochemistry: PUS1 Antibody [NB100-86983]

Immunohistochemistry: PUS1 Antibody [NB100-86983] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PUS1 used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB

Applications for PUS1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:250

Immunohistochemistry-Paraffin

1:250

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PUS1

PUS1 converts uridine into pseudouridine after the nucleotide has been incorporated into RNA. Pseudouridine may have a functional role in tRNAs and may assist in the peptidyl transfer reaction of rRNAs (Chen and Patton, 1999 [PubMed 10094309]).[supplied by OMIM]

Alternate Names

EC 5.4.99.12, MGC11268, mitochondrial tRNA pseudouridine synthase A, MLASA1, pseudouridylate synthase 1, tRNA pseudouridine synthase A, mitochondrial, tRNA pseudouridylate synthase I, tRNA uridine isomerase I, tRNA-uridine isomerase I

Entrez Gene IDs

80324 (Human)

Gene Symbol

PUS1

UniProt

NP_079491.2 (Human)

Additional PUS1 Products

Product Documents for PUS1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PUS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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