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PUS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32902

Novus Biologicals, part of Bio-Techne
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NBP1-32902

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PUS1. The exact sequence is proprietary.

Reactivity Notes

Sheep (87%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PUS1 Antibody

Western Blot: PUS1 Antibody [NBP1-32902]

Western Blot: PUS1 Antibody [NBP1-32902]

Western Blot: PUS1 Antibody [NBP1-32902] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: PUS1 Antibody [NBP1-32902]

Immunohistochemistry-Paraffin: PUS1 Antibody [NBP1-32902]

Immunohistochemistry-Paraffin: PUS1 Antibody [NBP1-32902] - Paraffin-embedded mouse muscle. PUS1 antibody [N2C3] diluted at 1:500.
Western Blot: PUS1 Antibody [NBP1-32902]

Western Blot: PUS1 Antibody [NBP1-32902]

Western Blot: PUS1 Antibody [NBP1-32902] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.

Applications for PUS1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PUS1

PUS1 converts uridine into pseudouridine after the nucleotide has been incorporated into RNA. Pseudouridine may have a functional role in tRNAs and may assist in the peptidyl transfer reaction of rRNAs (Chen and Patton, 1999 [PubMed 10094309]).[supplied by OMIM]

Alternate Names

EC 5.4.99.12, MGC11268, mitochondrial tRNA pseudouridine synthase A, MLASA1, pseudouridylate synthase 1, tRNA pseudouridine synthase A, mitochondrial, tRNA pseudouridylate synthase I, tRNA uridine isomerase I, tRNA-uridine isomerase I

Gene Symbol

PUS1

Additional PUS1 Products

Product Documents for PUS1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PUS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.