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PUS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13831

Novus Biologicals, part of Bio-Techne
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NBP2-13831

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for PUS1 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: PQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTEKVDVPKAPGLGLVLERVHFEKYNQRFG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PUS1 Antibody

Western Blot: PUS1 Antibody [NBP2-13831]

Western Blot: PUS1 Antibody [NBP2-13831]

Western Blot: PUS1 Antibody [NBP2-13831] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: PUS1 Antibody [NBP2-13831]

Immunohistochemistry-Paraffin: PUS1 Antibody [NBP2-13831]

Immunohistochemistry-Paraffin: PUS1 Antibody [NBP2-13831] - Staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells.

Applications for PUS1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PUS1

PUS1 converts uridine into pseudouridine after the nucleotide has been incorporated into RNA. Pseudouridine may have a functional role in tRNAs and may assist in the peptidyl transfer reaction of rRNAs (Chen and Patton, 1999 [PubMed 10094309]).[supplied by OMIM]

Alternate Names

EC 5.4.99.12, MGC11268, mitochondrial tRNA pseudouridine synthase A, MLASA1, pseudouridylate synthase 1, tRNA pseudouridine synthase A, mitochondrial, tRNA pseudouridylate synthase I, tRNA uridine isomerase I, tRNA-uridine isomerase I

Gene Symbol

PUS1

Additional PUS1 Products

Product Documents for PUS1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PUS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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