PYCR1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20016

Novus Biologicals, part of Bio-Techne
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NBP2-20016
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (93%), Mouse (92%), Rat (92%), Rhesus Macaque (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PYCR1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PYCR1. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (86%), Xenopus laevis (85%).

Localization

Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PYCR1 Antibody

Immunocytochemistry/ Immunofluorescence: PYCR1 Antibody [NBP2-20016]

Immunocytochemistry/ Immunofluorescence: PYCR1 Antibody [NBP2-20016]

Immunocytochemistry/Immunofluorescence: PYCR1 Antibody [NBP2-20016] - HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37- for 30 min.Green: PYCR1 protein stained by PYCR1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: PYCR1 Antibody [NBP2-20016]

Immunohistochemistry-Paraffin: PYCR1 Antibody [NBP2-20016]

Immunohistochemistry-Paraffin: PYCR1 Antibody [NBP2-20016] - Human breast cancer, using PYCR1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
PYCR1 Antibody

Western Blot: PYCR1 Antibody [NBP2-20016] -

Western Blot: PYCR1 Antibody [NBP2-20016] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with PYCR1 antibody (NBP2-20016) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for PYCR1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PYCR1

This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternate splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Alternate Names

ARCL2B, EC 1.5.1.2, mitochondrial pyrroline-5-carboxylate reductase 1, P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PRO3, proliferation-inducing protein 45, PYCR, pyrroline-5-carboxylate reductase 1, pyrroline-5-carboxylate reductase 1, mitochondrial

Gene Symbol

PYCR1

UniProt

P32322 (Human)

Additional PYCR1 Products

Product Documents for PYCR1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PYCR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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