PYGL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97708

Novus Biologicals, part of Bio-Techne
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NBP2-97708-100ul
NBP2-97708-50ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PYGL Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human PYGL fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PYGL Antibody

Immunocytochemistry/ Immunofluorescence: PYGL Antibody [NBP2-97708]

Immunocytochemistry/ Immunofluorescence: PYGL Antibody [NBP2-97708]

Immunocytochemistry/Immunofluorescence: PYGL Antibody [NBP2-97708] - Immunofluorescence staining of PYGL in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PYGL polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.
Immunohistochemistry-Paraffin: PYGL Antibody [NBP2-97708]

Immunohistochemistry-Paraffin: PYGL Antibody [NBP2-97708]

Immunohistochemistry-Paraffin: PYGL Antibody [NBP2-97708] - Immunochemical staining of human PYGL in human liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.

Applications for PYGL Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PYGL

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Activity of phosphorylase is controlled both by allosteric means (through the noncovalent binding of metabolites) and by covalent modification. Thus AMP allosterically activates, whereas ATP, ADP, and glucose 6 phosphate allosterically inhibit, phosphorylase B.

Long Name

Glycogen phosphorylase, liver form

Alternate Names

EC 2.4.1.1, glycogen phosphorylase, liver form, GSD6, phosphorylase, glycogen, liver, phosphorylase, glycogen; liver

Gene Symbol

PYGL

Additional PYGL Products

Product Documents for PYGL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PYGL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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