Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (SR1677)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-22675

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-22675-100ul
NBP3-22675-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1677

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Pyruvate Dehydrogenase E1-alpha subunit Antibodies »

Product Summary for Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (SR1677)

Immunogen

A synthesized peptide derived from human phospho-Pyruvate Dehydrogenase E1-alpha subunit [Ser293] (Uniprot #: P08559)

Modification

p Ser293

Specificity

Detects endogenous levels of total phospho-Pyruvate Dehydrogenase E1-alpha subunit [Ser293]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (SR1677)

Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (SR1677)

Western Blot-NBP3-22675-Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (SR1677)-

Western Blot-NBP3-22675-Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] -Antibody (SR1677)-Analysis of Phospho-PDHA1 (S293) expression in 293T cell lysate.

Applications for Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (SR1677)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pyruvate Dehydrogenase E1-alpha subunit

Pyruvate Dehydrogenase E1-alpha subunit (PDHE1 alpha) is a member of the pyruvate dehydrogenase complex (PDC) and is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1 or PDHE1), dihydrolipoyl transacetylase (DLAT), and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex. Enhanced expression of pyruvate dehydrogenase kinase-1 (PDK-1) results in phosphorylation of PDHE1 alpha at Serine 293, which in turn produces nearly complete inhibition of the pyruvate dehydrogenase complex (PDC). A recent study shows that inhibition of PDC activity in cancer cells restores normoxic stablization of HIF-1 alpha by glycolytic metabolites.

Alternate Names

EC 1.2.4.1, PDHA, PDHE1-A type I, PHE1APDHCE1A, pyruvate dehydrogenase (lipoamide) alpha 1, pyruvate dehydrogenase complex, E1-alpha polypeptide 1, pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Gene Symbol

PDHA1

Additional Pyruvate Dehydrogenase E1-alpha subunit Products

Product Documents for Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (SR1677)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (SR1677)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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