Pyruvate Dehydrogenase E1 beta subunit Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87421

Novus Biologicals, part of Bio-Techne
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NBP1-87421
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Pyruvate Dehydrogenase E1 beta subunit Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MAGLRPICEFMTFNFSMQAIDQVINSAAKTYYMSGGLQPVPIVFRGPNGASAGVAAQHSQCFAAWYGHCPGLKVVSPWNSEDAKG

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pyruvate Dehydrogenase E1 beta subunit Antibody

Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421]

Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421]

Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Analysis using Anti-PDHB antibody NBP1-87421 (A) shows similar pattern to independent antibody NBP2-38327 (B).
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in distal tubules.
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining of human heart muscle shows high expression.
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Applications for Pyruvate Dehydrogenase E1 beta subunit Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:10-1:20

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pyruvate Dehydrogenase E1 beta subunit

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3)

Alternate Names

DKFZp564K0164, mitochondrial, pyruvate dehydrogenase (lipoamide) beta, pyruvate dehydrogenase, E1 beta polypeptide

Gene Symbol

PDHB

Additional Pyruvate Dehydrogenase E1 beta subunit Products

Product Documents for Pyruvate Dehydrogenase E1 beta subunit Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Pyruvate Dehydrogenase E1 beta subunit Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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