Pyruvate Dehydrogenase E2 Antibody (2Z7R10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16707
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2Z7R10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Pyruvate Dehydrogenase E2 Antibody (2Z7R10)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 548-647 of human Pyruvate Dehydrogenase E2 (P10515). AREGKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLVPADNEKGFDVASMMSVTLSCDHRVVDGAVGAQWLAEFRKYLEKPITMLL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Pyruvate Dehydrogenase E2 Antibody (2Z7R10)
![Western Blot: Pyruvate Dehydrogenase E2 Antibody (2Z7R10) [NBP3-16707]](https://resources.bio-techne.com/images/products/Pyruvate-Dehydrogenase-E2-Antibody-2Z7R10-Western-Blot-NBP3-16707-img0001.jpg "Western Blot: Pyruvate Dehydrogenase E2 Antibody (2Z7R10) [NBP3-16707]")
Western Blot: Pyruvate Dehydrogenase E2 Antibody (2Z7R10) [NBP3-16707]
Western Blot: Pyruvate Dehydrogenase E2 Antibody (2Z7R10) [NBP3-16707] - Western blot analysis of extracts of various cell lines, using Pyruvate Dehydrogenase E2 Rabbit mAb (NBP3-16707) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.Applications for Pyruvate Dehydrogenase E2 Antibody (2Z7R10)
Application
Recommended Usage
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Pyruvate Dehydrogenase E2
Alternate Names
70 kDa mitochondrial autoantigen of primary biliary cirrhosis, dihydrolipoamide S-acetyltransferase, DLTA, E2 component of pyruvate dehydrogenase complex, EC 2.3.1, M2 antigen complex 70 kDa subunit, PBC, PDC-E2EC 2.3.1.12, PDCE2mitochondrial
Gene Symbol
DLAT
Additional Pyruvate Dehydrogenase E2 Products
Product Documents for Pyruvate Dehydrogenase E2 Antibody (2Z7R10)
Product Specific Notices for Pyruvate Dehydrogenase E2 Antibody (2Z7R10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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