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Pyruvate Dehydrogenase E2 Antibody (SR2133)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-22153

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-22153-100ul
NBP3-22153-50ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2133

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human Pyruvate Dehydrogenase E2 (Uniprot #: P10515)

Specificity

Detects endogenous levels of total Pyruvate Dehydrogenase E2

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Pyruvate Dehydrogenase E2 Antibody (SR2133)

Western Blot: Pyruvate Dehydrogenase E2 Antibody (SR2133) [NBP3-22153] -

Western Blot: Pyruvate Dehydrogenase E2 Antibody (SR2133) [NBP3-22153] - Western blot analysis using NBP3-22153 in Jurkat cell lysate.

Applications for Pyruvate Dehydrogenase E2 Antibody (SR2133)

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pyruvate Dehydrogenase E2

The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC; EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC; MIM 109720) show autoantibodies to DLAT.[supplied by OMIM]

Alternate Names

70 kDa mitochondrial autoantigen of primary biliary cirrhosis, dihydrolipoamide S-acetyltransferase, DLTA, E2 component of pyruvate dehydrogenase complex, EC 2.3.1, M2 antigen complex 70 kDa subunit, PBC, PDC-E2EC 2.3.1.12, PDCE2mitochondrial

Gene Symbol

DLAT

Additional Pyruvate Dehydrogenase E2 Products

Product Documents for Pyruvate Dehydrogenase E2 Antibody (SR2133)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Pyruvate Dehydrogenase E2 Antibody (SR2133)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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