Pyruvate Dehydrogenase E2 Antibody (SR2133)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-22153
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SR2133
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Pyruvate Dehydrogenase E2 Antibody (SR2133)
Immunogen
A synthesized peptide derived from human Pyruvate Dehydrogenase E2 (Uniprot #: P10515)
Specificity
Detects endogenous levels of total Pyruvate Dehydrogenase E2
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Pyruvate Dehydrogenase E2 Antibody (SR2133)
Western Blot: Pyruvate Dehydrogenase E2 Antibody (SR2133) [NBP3-22153] -
Western Blot: Pyruvate Dehydrogenase E2 Antibody (SR2133) [NBP3-22153] - Western blot analysis using NBP3-22153 in Jurkat cell lysate.Applications for Pyruvate Dehydrogenase E2 Antibody (SR2133)
Application
Recommended Usage
Flow Cytometry
1:50
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunoprecipitation
1:50
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Pyruvate Dehydrogenase E2
Alternate Names
70 kDa mitochondrial autoantigen of primary biliary cirrhosis, dihydrolipoamide S-acetyltransferase, DLTA, E2 component of pyruvate dehydrogenase complex, EC 2.3.1, M2 antigen complex 70 kDa subunit, PBC, PDC-E2EC 2.3.1.12, PDCE2mitochondrial
Gene Symbol
DLAT
Additional Pyruvate Dehydrogenase E2 Products
Product Documents for Pyruvate Dehydrogenase E2 Antibody (SR2133)
Product Specific Notices for Pyruvate Dehydrogenase E2 Antibody (SR2133)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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