Pyruvate Dehydrogenase E2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-54756
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.5 mg/ml
Product Summary for Pyruvate Dehydrogenase E2 Antibody
Immunogen
Synthetic peptides corresponding to DLAT(dihydrolipoamide S-acetyltransferase) The peptide sequence was selected from the C terminal of DLAT.
Peptide sequence DVVSLATKAREGKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILA. The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for Pyruvate Dehydrogenase E2 Antibody
![Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54756]](https://resources.bio-techne.com/images/products/Pyruvate-Dehydrogenase-E2-Antibody-Western-Blot-NBP1-54756-img0003.jpg "Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54756]")
Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54756]
Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54756] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.Applications for Pyruvate Dehydrogenase E2 Antibody
Application
Recommended Usage
Western Blot
1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Pyruvate Dehydrogenase E2
Alternate Names
70 kDa mitochondrial autoantigen of primary biliary cirrhosis, dihydrolipoamide S-acetyltransferase, DLTA, E2 component of pyruvate dehydrogenase complex, EC 2.3.1, M2 antigen complex 70 kDa subunit, PBC, PDC-E2EC 2.3.1.12, PDCE2mitochondrial
Gene Symbol
DLAT
UniProt
Additional Pyruvate Dehydrogenase E2 Products
Product Documents for Pyruvate Dehydrogenase E2 Antibody
Product Specific Notices for Pyruvate Dehydrogenase E2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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