Pyruvate Dehydrogenase E2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-54795

Novus Biologicals, part of Bio-Techne
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NBP1-54795
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

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Product Summary for Pyruvate Dehydrogenase E2 Antibody

Immunogen

Synthetic peptides corresponding to DLAT(dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex)) The peptide sequence was selected from the N terminal of DLAT. Peptide sequence WTPSSGATPRNRLLLQLLGSPGRRYYSLPPHQKVPLPSLSPTMQAGTIAR The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Pyruvate Dehydrogenase E2 Antibody

Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54795]

Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54795]

Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54795] - Reccomended Titration: 5.0 ug/ml Positive Control: Jurkat cell lysate
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP1-54795]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP1-54795]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP1-54795] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54795]

Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54795]

Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP1-54795] - Sample Type : Huh7 lysate (50ug) Primary Antibody Dilution : 1:200 Image Submitted By : Partha Kasturi University of Kansas Medical Center
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Applications for Pyruvate Dehydrogenase E2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pyruvate Dehydrogenase E2

DLAT is dihydrolipoamide acetyltransferase, the E2 subunit of the mammalian pyruvate dehydrogenase complex of the inner mitochondrial membrane. Patients with primary biliary cirrhosis show autoantibodies to DLAT.The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC; EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC; MIM 109720) show autoantibodies to DLAT.[supplied by OMIM].

Alternate Names

70 kDa mitochondrial autoantigen of primary biliary cirrhosis, dihydrolipoamide S-acetyltransferase, DLTA, E2 component of pyruvate dehydrogenase complex, EC 2.3.1, M2 antigen complex 70 kDa subunit, PBC, PDC-E2EC 2.3.1.12, PDCE2mitochondrial

Gene Symbol

DLAT

UniProt

Q86YI5 (Human)

Additional Pyruvate Dehydrogenase E2 Products

Product Documents for Pyruvate Dehydrogenase E2 Antibody

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Certificate of Analysis

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Product Specific Notices for Pyruvate Dehydrogenase E2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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