Pyruvate Dehydrogenase E2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20026

Novus Biologicals, part of Bio-Techne
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NBP2-20026
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Pyruvate Dehydrogenase E2 Antibodies »

Product Summary for Pyruvate Dehydrogenase E2 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pyruvate Dehydrogenase E2. The exact sequence is proprietary.

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Pyruvate Dehydrogenase E2 Antibody

Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026]

Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026]

Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026]

Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026]

Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026] - DLAT antibody detects DLAT protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DLAT protein stained by DLAT antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026] - Immunohistochemical analysis of paraffin-embedded Ovarian carcinoma, using antibody at 1:250 dilution.

Applications for Pyruvate Dehydrogenase E2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Pyruvate Dehydrogenase E2

The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC; EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC; MIM 109720) show autoantibodies to DLAT.[supplied by OMIM]

Alternate Names

70 kDa mitochondrial autoantigen of primary biliary cirrhosis, dihydrolipoamide S-acetyltransferase, DLTA, E2 component of pyruvate dehydrogenase complex, EC 2.3.1, M2 antigen complex 70 kDa subunit, PBC, PDC-E2EC 2.3.1.12, PDCE2mitochondrial

Gene Symbol

DLAT

UniProt

P10515 (Human)

Additional Pyruvate Dehydrogenase E2 Products

Product Documents for Pyruvate Dehydrogenase E2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Pyruvate Dehydrogenase E2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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