Pyruvate Dehydrogenase E2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20026
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Pyruvate Dehydrogenase E2. The exact sequence is proprietary.
Localization
Mitochondrion matrix
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Pyruvate Dehydrogenase E2 Antibody
![Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026]](https://resources.bio-techne.com/images/products/Pyruvate-Dehydrogenase-E2-Antibody-Western-Blot-NBP2-20026-img0001.jpg "Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026]")
Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026]
Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7. 5% SDS PAGE gel, diluted at 1:10000.![Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026]](https://resources.bio-techne.com/images/products/Pyruvate-Dehydrogenase-E2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20026-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026]")
Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026]
Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026] - DLAT antibody detects DLAT protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DLAT protein stained by DLAT antibody diluted at 1:500. Blue: Hoechst 33342 staining.![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026]](https://resources.bio-techne.com/images/products/Pyruvate-Dehydrogenase-E2-Antibody-Immunohistochemistry-Paraffin-NBP2-20026-img0002.jpg "Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026]")
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026]
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-20026] - Immunohistochemical analysis of paraffin-embedded Ovarian carcinoma, using antibody at 1:250 dilution.Applications for Pyruvate Dehydrogenase E2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:5000-1:20000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Pyruvate Dehydrogenase E2
Alternate Names
70 kDa mitochondrial autoantigen of primary biliary cirrhosis, dihydrolipoamide S-acetyltransferase, DLTA, E2 component of pyruvate dehydrogenase complex, EC 2.3.1, M2 antigen complex 70 kDa subunit, PBC, PDC-E2EC 2.3.1.12, PDCE2mitochondrial
Gene Symbol
DLAT
UniProt
Additional Pyruvate Dehydrogenase E2 Products
Product Documents for Pyruvate Dehydrogenase E2 Antibody
Product Specific Notices for Pyruvate Dehydrogenase E2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.