Pyruvate Dehydrogenase E2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99340

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Pyruvate Dehydrogenase E2 Antibodies »

Product Summary for Pyruvate Dehydrogenase E2 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Pyruvate Dehydrogenase E2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Pyruvate Dehydrogenase E2 Antibody

Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340]

Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340]

Western Blot: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340] - Anti-Pyruvate Dehydrogenase E2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 72 kDa
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340] - Immunochemical staining of human Pyruvate Dehydrogenase E2 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340]

Immunoprecipitation: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340]

Immunoprecipitation: Pyruvate Dehydrogenase E2 Antibody [NBP2-99340] - Pyruvate Dehydrogenase E2 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 2 ul anti-Pyruvate Dehydrogenase E2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Pyruvate Dehydrogenase E2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 72 kDa

Applications for Pyruvate Dehydrogenase E2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pyruvate Dehydrogenase E2

The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC; EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC; MIM 109720) show autoantibodies to DLAT.[supplied by OMIM]

Alternate Names

70 kDa mitochondrial autoantigen of primary biliary cirrhosis, dihydrolipoamide S-acetyltransferase, DLTA, E2 component of pyruvate dehydrogenase complex, EC 2.3.1, M2 antigen complex 70 kDa subunit, PBC, PDC-E2EC 2.3.1.12, PDCE2mitochondrial

Gene Symbol

DLAT

Additional Pyruvate Dehydrogenase E2 Products

Product Documents for Pyruvate Dehydrogenase E2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Pyruvate Dehydrogenase E2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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