Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93618

Novus Biologicals, part of Bio-Techne
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NBP2-93618-0.02ml
NBP2-93618-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Pyruvate Dehydrogenase Phosphatase/PDP1 Antibodies »

Product Summary

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 72-260 of human PDP1 (NP_060914.2). ASTPQKFYLTPPQVNSILKANEYSFKVPEFDGKNVSSILGFDSNQLPANAPIEDRRSAATCLQTRGMLLGVFDGHAGCACSQAVSERLFYYIAVSLLPHETLLEIENAVESGRALLPILQWHKHPNDYFSKEASKLYFNSLRTYWQELIDLNTGESTDIDVKEALINAFKRLDNDISLEAQVGDPNSFL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images

Western Blot: Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C AntibodyAzide and BSA Free [NBP2-93618]

Western Blot: Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C AntibodyAzide and BSA Free [NBP2-93618]

Western Blot: Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody [NBP2-93618] - Analysis of extracts of various cell lines, using Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody - Azide and BSA Free [NBP2-93618]

Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody - Azide and BSA Free [NBP2-93618]

Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody [NBP2-93618] - Analysis of L929 cells using PDP1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Pyruvate Dehydrogenase Phosphatase/PDP1

Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.(provided by RefSeq)

Alternate Names

PDH, PDP1, PDPC 1, PPM2C

Gene Symbol

PDP1

Additional Pyruvate Dehydrogenase Phosphatase/PDP1 Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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