Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93866

Novus Biologicals, part of Bio-Techne
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NBP2-93866-0.02ml
NBP2-93866-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Pyruvate Dehydrogenase Phosphatase/PDP1 Antibodies »

Product Summary for Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 278-537 of human PDP1 (NP_060914.2). HVDGVDLHVANTGDSRAMLGVQEEDGSWSAVTLSNDHNAQNERELERLKLEHPKSEAKSVVKQDRLLGLLMPFRAFGDVKFKWSIDLQKRVIESGPDQLNDNEYTKFIPPNYHTPPYLTAEPEVTYHRLRPQDKFLVLATDGLWETMHRQDVVRIVGEYLTGMHHQQPIAVGGYKVTLGQMHGLLTERRTKMSSVFEDQNAATHLIRHAVGNNEFGTVDHERLSKMLSLPEELARMYRDDITIIVVQFNSHVVGAYQNQE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody - BSA Free

Western Blot: Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C AntibodyBSA Free [NBP2-93866]

Western Blot: Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C AntibodyBSA Free [NBP2-93866]

Western Blot: Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody [NBP2-93866] - Analysis of extracts of various cell lines, using Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody - BSA Free [NBP2-93866]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody - BSA Free [NBP2-93866]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody [NBP2-93866] - Paraffin-embedded rat brain using Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C .
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody - BSA Free [NBP2-93866]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody - BSA Free [NBP2-93866]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody [NBP2-93866] - Paraffin-embedded mouse brain using Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C .
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Applications for Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Pyruvate Dehydrogenase Phosphatase/PDP1

Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.(provided by RefSeq)

Alternate Names

PDH, PDP1, PDPC 1, PPM2C

Gene Symbol

PDP1

Additional Pyruvate Dehydrogenase Phosphatase/PDP1 Products

Product Documents for Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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