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QARS Antibody (5F5)

Novus Biologicals, part of Bio-Techne | Catalog # H00005859-M01

Novus Biologicals, part of Bio-Techne
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H00005859-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 5F5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

QARS (NP_005042, 677 a.a. ~ 775 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FIHWVSQPLMCEVRLYERLFQHKNPEDPTEVPGGFLSDLNLASLHVVDAALVDCSVALAKPFDKFQFERLGYFSVDPDSHQGKLVFNRTVTLKEDPGKV

Specificity

QARS - glutaminyl-tRNA synthetase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for QARS Antibody (5F5)

Western Blot: QARS Antibody (5F5) [H00005859-M01]

Western Blot: QARS Antibody (5F5) [H00005859-M01]

Western Blot: QARS Antibody (5F5) [H00005859-M01] - Analysis of QARS expression in transfected 293T cell line by QARS monoclonal antibody (M01), clone 5F5.Lane 1: QARS transfected lysate(87.8 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: QARS Antibody (5F5) [H00005859-M01]

Immunohistochemistry-Paraffin: QARS Antibody (5F5) [H00005859-M01]

Immunohistochemistry-Paraffin: QARS Antibody (5F5) [H00005859-M01] - Analysis of monoclonal antibody to QARS on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Western Blot: QARS Antibody (5F5) [H00005859-M01]

Western Blot: QARS Antibody (5F5) [H00005859-M01]

Western Blot: QARS Antibody (5F5) [H00005859-M01] - QARS monoclonal antibody (M01), clone 5F5 Analysis of QARS expression in HeLa.

Applications for QARS Antibody (5F5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: QARS

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. In metazoans, 9 aminoacyl-tRNA synthetases specific for glutamine (gln), glutamic acid (glu), and 7 other amino acids are associated within a multienzyme complex. Although present in eukaryotes, glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family.

Alternate Names

EC 6.1.1, EC 6.1.1.18, GLNRS, glutamine tRNA ligase, Glutamine--tRNA ligase, glutamine-tRNA synthetase, glutaminyl-tRNA synthetase, PRO2195

Entrez Gene IDs

5859 (Human)

Gene Symbol

QARS1

OMIM

603727 (Human)

Additional QARS Products

Product Documents for QARS Antibody (5F5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for QARS Antibody (5F5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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