QKI/Quaking Antibody (1D1N6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15255
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1D1N6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 242-341 of human QKI/Quaking (Q96PU8). RTPTPAGPTIMPLIRQIQTAVMPNGTPHPTAAIVPPGPEAGLIYTPYEYPYTLAPATSILEYPIEPSGVLGAVATKVRRHDMRVHPYQRIVTADRAATGN
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for QKI/Quaking Antibody (1D1N6)
![Western Blot: QKI/Quaking Antibody (1D1N6) [NBP3-15255]](https://resources.bio-techne.com/images/products/QKI-Quaking-Antibody-1D1N6-Western-Blot-NBP3-15255-img0002.jpg "Western Blot: QKI/Quaking Antibody (1D1N6) [NBP3-15255]")
Western Blot: QKI/Quaking Antibody (1D1N6) [NBP3-15255]
Western Blot: QKI/Quaking Antibody (1D1N6) [NBP3-15255] - Western blot analysis of extracts of various cell lines, using QKI/Quaking antibody (NBP3-15255) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody (1D1N6) [NBP3-15255]](https://resources.bio-techne.com/images/products/QKI-Quaking-Antibody-1D1N6-Immunocytochemistry-Immunofluorescence-NBP3-15255-img0001.jpg "Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody (1D1N6) [NBP3-15255]")
Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody (1D1N6) [NBP3-15255]
Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (1D1N6) [NBP3-15255] - Immunofluorescence analysis of NIH/3T3 cells using QKI/Quaking Rabbit mAb (NBP3-15255) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.![Immunoprecipitation: QKI/Quaking Antibody (1D1N6) [NBP3-15255]](https://resources.bio-techne.com/images/products/QKI-Quaking-Antibody-1D1N6-Immunoprecipitation-NBP3-15255-img0003.jpg "Immunoprecipitation: QKI/Quaking Antibody (1D1N6) [NBP3-15255]")
Immunoprecipitation: QKI/Quaking Antibody (1D1N6) [NBP3-15255]
Immunoprecipitation: QKI/Quaking Antibody (1D1N6) [NBP3-15255] - Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug QKI/Quaking antibody (NBP3-15255). Western blot was performed from the immunoprecipitate using QKI/Quaking antibody (NBP3-15255) at a dilition of 1:1000.Applications for QKI/Quaking Antibody (1D1N6)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: QKI/Quaking
Alternate Names
DKFZp586I0923, HKQ, homolog of mouse quaking QKI (KH domain RNA binding protein), Hqk, hqkI, protein quaking, QK, QK1, QK3, quaking homolog, KH domain RNA binding (mouse), RNA binding protein HQK
Gene Symbol
QKI
Additional QKI/Quaking Products
Product Documents for QKI/Quaking Antibody (1D1N6)
Product Specific Notices for QKI/Quaking Antibody (1D1N6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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