QKI/Quaking Antibody (1D1N6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15255

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15255-20ul
NBP3-15255-100ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1D1N6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all QKI/Quaking Antibodies »

Product Summary for QKI/Quaking Antibody (1D1N6)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 242-341 of human QKI/Quaking (Q96PU8). RTPTPAGPTIMPLIRQIQTAVMPNGTPHPTAAIVPPGPEAGLIYTPYEYPYTLAPATSILEYPIEPSGVLGAVATKVRRHDMRVHPYQRIVTADRAATGN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for QKI/Quaking Antibody (1D1N6)

Western Blot: QKI/Quaking Antibody (1D1N6) [NBP3-15255]

Western Blot: QKI/Quaking Antibody (1D1N6) [NBP3-15255]

Western Blot: QKI/Quaking Antibody (1D1N6) [NBP3-15255] - Western blot analysis of extracts of various cell lines, using QKI/Quaking antibody (NBP3-15255) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody (1D1N6) [NBP3-15255]

Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody (1D1N6) [NBP3-15255]

Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (1D1N6) [NBP3-15255] - Immunofluorescence analysis of NIH/3T3 cells using QKI/Quaking Rabbit mAb (NBP3-15255) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation: QKI/Quaking Antibody (1D1N6) [NBP3-15255]

Immunoprecipitation: QKI/Quaking Antibody (1D1N6) [NBP3-15255]

Immunoprecipitation: QKI/Quaking Antibody (1D1N6) [NBP3-15255] - Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug QKI/Quaking antibody (NBP3-15255). Western blot was performed from the immunoprecipitate using QKI/Quaking antibody (NBP3-15255) at a dilition of 1:1000.

Applications for QKI/Quaking Antibody (1D1N6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: QKI/Quaking

QKI belongs to a family of RNA-binding proteins that have an HNRNPK (MIM 600712) homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1; MIM 602489) and SF1 (MIM 601516) (Chen and Richard, 1998).[supplied by OMIM]

Alternate Names

DKFZp586I0923, HKQ, homolog of mouse quaking QKI (KH domain RNA binding protein), Hqk, hqkI, protein quaking, QK, QK1, QK3, quaking homolog, KH domain RNA binding (mouse), RNA binding protein HQK

Gene Symbol

QKI

Additional QKI/Quaking Products

Product Documents for QKI/Quaking Antibody (1D1N6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for QKI/Quaking Antibody (1D1N6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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