Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # S147-6

Concentration

1 mg/ml

View all QKI/Quaking Antibodies »

Product Summary for QKI/Quaking Antibody (S147-6) - Pan Specific

Immunogen

Fusion protein amino acids 1-341 (full-length) of human QKI-5. Mouse: 100% identity (341/341 amino acids identical). Rat: 99% identity (339/341 amino acids identical) >90% identity with QKI-6, QKI-7 and QKI-7b.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Mouse and Rat

Localization

Cytoplasm , Nucleus

Specificity

Greater then 90% identity with QKI-6, QKI-7 and QKI-7b.Detects approx 36-38kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for QKI/Quaking Antibody (S147-6) - Pan Specific

Western Blot: QKI/Quaking Antibody (S147-6)Pan Specific [NBP2-42196]

Western Blot: QKI/Quaking Antibody (S147-6)Pan Specific [NBP2-42196]

Western Blot: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196] - Western Blot analysis of Rat Brain Membrane showing detection of 36-38 kDa QKI/Quaking (pan) protein using Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody, Clone S147-6 (NBP2-42196). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody (NBP2-42196) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 36-38 kDa.
Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196]

Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196]

Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody, Clone S147-6 (NBP2-42196). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-QKI/Quaking (pan) Monoclonal Antibody (NBP2-42196) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) QKI/Quaking (pan) Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196]

Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196]

Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (S147-6) - Pan Specific [NBP2-42196] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-QKI (pan) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.

Applications for QKI/Quaking Antibody (S147-6) - Pan Specific

Application
Recommended Usage

Western Blot

1:1000
Application Notes
1 ug/ml of QKI (pan) Antibody was sufficient for detection of Pan-QKI in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: QKI/Quaking

QKI belongs to a family of RNA-binding proteins that have an HNRNPK (MIM 600712) homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1; MIM 602489) and SF1 (MIM 601516) (Chen and Richard, 1998).[supplied by OMIM]

Alternate Names

DKFZp586I0923, HKQ, homolog of mouse quaking QKI (KH domain RNA binding protein), Hqk, hqkI, protein quaking, QK, QK1, QK3, quaking homolog, KH domain RNA binding (mouse), RNA binding protein HQK

Gene Symbol

QKI

Additional QKI/Quaking Products

Product Documents for QKI/Quaking Antibody (S147-6) - Pan Specific

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for QKI/Quaking Antibody (S147-6) - Pan Specific

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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