QPRT Antibody (5D11)
Novus Biologicals, part of Bio-Techne | Catalog # H00023475-M01
Key Product Details
Species Reactivity
Validated:
Human, Avian
Cited:
Avian
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5D11
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
QPRT (NP_055113, 198 a.a. ~ 297 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VEVECSSLQEAVQAAEAGADLVLLDNFKPEELHPTATVLKAQFPSVAVEASGGITLDNLPQFCGPHIDVISMGMLTQAAPALDFSLKLFAKEVAPVPKIH
Reactivity Notes
Human. Other species not tested. Avian reactivity reported in scientific literature (PMID: 23761975)
Specificity
QPRT - quinolinate phosphoribosyltransferase (nicotinate-nucleotide pyrophosphorylase (carboxylating))
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for QPRT Antibody (5D11)
![Western Blot: QPRT Antibody (5D11) [H00023475-M01]](https://resources.bio-techne.com/images/products/QPRT-Antibody-5D11-Western-Blot-H00023475-M01-img0007.jpg "Western Blot: QPRT Antibody (5D11) [H00023475-M01]")
Western Blot: QPRT Antibody (5D11) [H00023475-M01]
Western Blot: QPRT Antibody (5D11) [H00023475-M01] - QPRT monoclonal antibody (M01), clone 5D11 Analysis of QPRT expression in HepG2.![Immunohistochemistry-Paraffin: QPRT Antibody (5D11) [H00023475-M01]](https://resources.bio-techne.com/images/products/QPRT-Antibody-5D11-Immunohistochemistry-Paraffin-H00023475-M01-img0005.jpg "Immunohistochemistry-Paraffin: QPRT Antibody (5D11) [H00023475-M01]")
Immunohistochemistry-Paraffin: QPRT Antibody (5D11) [H00023475-M01]
Immunohistochemistry-Paraffin: QPRT Antibody (5D11) [H00023475-M01] - Analysis of monoclonal antibody to QPRT on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Sandwich ELISA: QPRT Antibody (5D11) [H00023475-M01] - Detection limit for recombinant GST tagged QPRT is approximately 0.03ng/ml as a capture antibody.
Applications for QPRT Antibody (5D11)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: QPRT
Alternate Names
EC 2.4.2.19, nicotinate-nucleotide pyrophosphorylase (carboxylating), QAPRTase, QPRTasenicotinate-nucleotide pyrophosphorylase [carboxylating], quinolinate phosphoribosyltransferase, Quinolinate phosphoribosyltransferase [decarboxylating]
Entrez Gene IDs
23475 (Human)
Gene Symbol
QPRT
OMIM
606248 (Human)
UniProt
Additional QPRT Products
Product Documents for QPRT Antibody (5D11)
Product Specific Notices for QPRT Antibody (5D11)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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