QTRT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20030

Novus Biologicals, part of Bio-Techne
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NBP2-20030
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all QTRT1 Antibodies »

Product Summary for QTRT1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human QTRT1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (83%), Zebrafish (81%), Xenopus tropicalis (83%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for QTRT1 Antibody

Western Blot: QTRT1 Antibody [NBP2-20030]

Western Blot: QTRT1 Antibody [NBP2-20030]

Western Blot: QTRT1 Antibody [NBP2-20030] - Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: QTRT1 Antibody [NBP2-20030]

Immunocytochemistry/ Immunofluorescence: QTRT1 Antibody [NBP2-20030]

Immunocytochemistry/Immunofluorescence: QTRT1 Antibody [NBP2-20030] - QTRT1 antibody detects QTRT1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: QTRT1 protein stained by QTRT1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: QTRT1 Antibody [NBP2-20030]

Immunohistochemistry-Paraffin: QTRT1 Antibody [NBP2-20030]

Immunohistochemistry-Paraffin: QTRT1 Antibody [NBP2-20030] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.
View 1 more images

Applications for QTRT1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: QTRT1

tRNA-guanine transglycosylase (TGT; EC 2.4.2.29) synthesizes queuosine (Q), which is found in tRNAs that recognize NAU and NAC codons, encoding tyr, asn, asp, and his. Prokaryotic TGT is a single protein of 43 kD. In contrast, mammalian TGT appears to be a heterodimer consisting of a 60-kD subunit (USP14; MIM 607274) and a 43-kD catalytic subunit (QTRT1) (Deshpande and Katze, 2001 [PubMed 11255023]).[supplied by OMIM]

Alternate Names

EC 2.4.2.29, Guanine insertion enzyme, queuine tRNA-ribosyltransferase, queuine tRNA-ribosyltransferase 1, TGTFP3235, TGUT, tRNA-guanine transglycosylase

Gene Symbol

QTRT1

UniProt

Q9BXR0 (Human)

Additional QTRT1 Products

Product Documents for QTRT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for QTRT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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