RAB10 Antibody (4E2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51634

Novus Biologicals, part of Bio-Techne
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NBP1-51634
NBP1-51634-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4E2

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all RAB10 Antibodies »

Product Summary for RAB10 Antibody (4E2) - BSA Free

Immunogen

Purified recombinant fragment of human Rab10 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RAB10 Antibody (4E2) - BSA Free

Western Blot: RAB10 Antibody (4E2)BSA Free [NBP1-51634]

Western Blot: RAB10 Antibody (4E2)BSA Free [NBP1-51634]

Western Blot: RAB10 Antibody (4E2) [NBP1-51634] - Analysis using Rab10 mouse mAb against Hela (1) and NIH/3T3 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: RAB10 Antibody (4E2) - BSA Free [NBP1-51634]

Immunocytochemistry/ Immunofluorescence: RAB10 Antibody (4E2) - BSA Free [NBP1-51634]

Immunocytochemistry/Immunofluorescence: RAB10 Antibody (4E2) [NBP1-51634] - Immunofluorescence analysis of LOVO cells using Rab10 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Applications for RAB10 Antibody (4E2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAB10

RAB10, member RAS oncogene family. The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway.

Alternate Names

GTP-binding protein RAB10, RAB10, member RAS oncogene family, ras-related GTP-binding protein, ras-related protein Rab-10

Gene Symbol

RAB10

Additional RAB10 Products

Product Documents for RAB10 Antibody (4E2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAB10 Antibody (4E2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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