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Rab20 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20039

Novus Biologicals, part of Bio-Techne
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NBP2-20039

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Rab20 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAB20. The exact sequence is proprietary.

Localization

Golgi apparatus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rab20 Antibody

Western Blot: Rab20 Antibody [NBP2-20039]

Western Blot: Rab20 Antibody [NBP2-20039]

Western Blot: Rab20 Antibody [NBP2-20039] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB20 antibody [N1C3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Rab20 Antibody [NBP2-20039]

Immunocytochemistry/ Immunofluorescence: Rab20 Antibody [NBP2-20039]

Immunocytochemistry/Immunofluorescence: Rab20 Antibody [NBP2-20039] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Rab20 Antibody [NBP2-20039]

Immunohistochemistry-Paraffin: Rab20 Antibody [NBP2-20039]

Immunohistochemistry-Paraffin: Rab20 Antibody [NBP2-20039] - Human hepatocellular carcinomas. RAB20 antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for Rab20 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rab20

Plays a role in apical endocytosis/recycling.

Alternate Names

FLJ20429, RAB20, member RAS oncogene family, ras-related protein Rab-20

Gene Symbol

RAB20

UniProt

Additional Rab20 Products

Product Documents for Rab20 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rab20 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.