Rab25 Antibody (3F12F3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28845

Novus Biologicals, part of Bio-Techne
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NBP1-28845-0.1ml
NBP1-28845-0.025ml
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 3F12F3

Format

BSA Free

Concentration

1 mg/ml

View all Rab25 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of Rab25 expressed in E. Coli.

Reactivity Notes

Human.

Specificity

Specific for Rab25.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Rab25 Antibody (3F12F3) - BSA Free

Western Blot: Rab25 Antibody (3F12F3)BSA Free [NBP1-28845]

Western Blot: Rab25 Antibody (3F12F3)BSA Free [NBP1-28845]

Western Blot: Rab25 Antibody (3F12F3) [NBP1-28845] - Western blot analysis using Rab25 mouse mAb against truncated Rab25 recombinant protein (1), human overy carcinoma(2), stomach carcinoma (3), breast carcinoma (4) tissue lysate.
Immunohistochemistry: Rab25 Antibody (3F12F3) - BSA Free [NBP1-28845]

Immunohistochemistry: Rab25 Antibody (3F12F3) - BSA Free [NBP1-28845]

Immunohistochemistry: Rab25 Antibody (3F12F3) [NBP1-28845] - analysis of paraffin-embedded human bladder carcinoma(A), breast carcinoma(B), esophagus carcinoma(C), skin carcinoma(D) tissue, showing cytoplasmic localization using anti-Rab25 antibody with DAB staining.

Applications for Rab25 Antibody (3F12F3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
ELISA, Western Blot, Immunohistochemistry-Paraffin and Immunohistochemistry-Frozen

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rab25

Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa

Long Name

RAs Genes from Brain Protein 25

Alternate Names

CATX8, RAB11C

Gene Symbol

RAB25

Additional Rab25 Products

Product Documents for Rab25 Antibody (3F12F3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rab25 Antibody (3F12F3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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