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RAB35 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-04511

Novus Biologicals, part of Bio-Techne
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NBP3-04511-100ul
NBP3-04511-20ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for RAB35 Antibody - Azide and BSA Free

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-201 of human RAB35 (NP_006852.1). KRWLHEINQNCDDVCRILVGNKNDDPERKVVETEDAYKFAGQMGIQLFETSAKENVNVEEMFNCITELVLRAKKDNLAKQQQQQQNDVVKLTKNSKRKKRCC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAB35 Antibody - Azide and BSA Free

Western Blot: RAB35 AntibodyAzide and BSA Free [NBP3-04511]

Western Blot: RAB35 AntibodyAzide and BSA Free [NBP3-04511]

Western Blot: RAB35 Antibody [NBP3-04511] - Western blot analysis of extracts of various cell lines, using RAB35 antibody (NBP3-04511) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: RAB35 Antibody - Azide and BSA Free [NBP3-04511]

Immunocytochemistry/ Immunofluorescence: RAB35 Antibody - Azide and BSA Free [NBP3-04511]

Immunocytochemistry/Immunofluorescence: RAB35 Antibody [NBP3-04511] - Immunofluorescence analysis of PC-12 cells using RAB35 Rabbit pAb (NBP3-04511) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: RAB35 Antibody - Azide and BSA Free [NBP3-04511]

Immunohistochemistry-Paraffin: RAB35 Antibody - Azide and BSA Free [NBP3-04511]

Immunohistochemistry-Paraffin: RAB35 Antibody [NBP3-04511] - Immunohistochemistry of paraffin-embedded rat heart using RAB35 Rabbit pAb (NBP3-04511) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for RAB35 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAB35

Possesses GTPase activity

Alternate Names

GTP-binding protein RAY, H-ray, RAB1C, RAB35, member RAS oncogene family, Ras-related protein Rab-1C, ras-related protein rab-1c (GTP-binding protein ray), ras-related protein Rab-35, RAY

Gene Symbol

RAB35

Additional RAB35 Products

Product Documents for RAB35 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RAB35 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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