Rab3C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32871

Novus Biologicals, part of Bio-Techne
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NBP1-32871
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Rab3C Antibodies »

Product Specifications

Immunogen

Full length human RAB3C Recombinant protein.

Localization

Cell membrane, Lipid-anchor, Cytoplasmic side

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rab3C Antibody

Western Blot: Rab3C Antibody [NBP1-32871]

Western Blot: Rab3C Antibody [NBP1-32871]

Western Blot: Rab3C Antibody [NBP1-32871] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAB3C antibody [N1C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Rab3C Antibody [NBP1-32871]

Immunocytochemistry/ Immunofluorescence: Rab3C Antibody [NBP1-32871]

Immunocytochemistry/Immunofluorescence: Rab3C Antibody [NBP1-32871] - DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAB3C stained by RAB3C antibody [N1C3] diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: Rab3C Antibody [NBP1-32871]

Immunohistochemistry-Paraffin: Rab3C Antibody [NBP1-32871]

Immunohistochemistry-Paraffin: Rab3C Antibody [NBP1-32871] - DLD1 xenograft, using RAB3C antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Applications for Rab3C Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rab3C

Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 Rab proteins that have been described in mammals. Rab 3C is found in brain, adrenal gland, and pituitary.

Alternate Names

RAB3C, member RAS oncogene family, ras-related protein Rab-3C

Gene Symbol

RAB3C

Additional Rab3C Products

Product Documents for Rab3C Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rab3C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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