Key Product Details

Species Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5F2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all RAB3IP Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RAB3IP (NP_071901) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

51.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RAB3IP Antibody (OTI5F2) - Azide and BSA Free

Western Blot: RAB3IP Antibody (OTI5F2)Azide and BSA Free [NBP2-73775]

Western Blot: RAB3IP Antibody (OTI5F2)Azide and BSA Free [NBP2-73775]

Western Blot: RAB3IP Antibody (OTI5F2) - Azide and BSA Free [NBP2-73775] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAB3IP .
Immunocytochemistry/ Immunofluorescence: RAB3IP Antibody (OTI5F2) - Azide and BSA Free [NBP2-73775]

Immunocytochemistry/ Immunofluorescence: RAB3IP Antibody (OTI5F2) - Azide and BSA Free [NBP2-73775]

Immunocytochemistry/Immunofluorescence: RAB3IP Antibody (OTI5F2) - Azide and BSA Free [NBP2-73775] - Zebrafish neuromast stained for Rab3IP (red) and counterstained with phalloidin (green). Image courtesy of customer.
Immunohistochemistry: RAB3IP Antibody (OTI5F2) - Azide and BSA Free [NBP2-73775]

Immunohistochemistry: RAB3IP Antibody (OTI5F2) - Azide and BSA Free [NBP2-73775]

Immunohistochemistry: RAB3IP Antibody (OTI5F2) - Azide and BSA Free [NBP2-73775] - Staining of paraffin-embedded Human colon tissue within the normal limits using anti-RAB3IP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3min) (1:150)
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Applications for RAB3IP Antibody (OTI5F2) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAB3IP

RAB3IP, also known as Rab-3A-interacting protein, is a 53 kDa 476 amino acid protein with 8 isoforms produced by alternative splicing. The primary function of RAB3IP is to change GDP proteins into GTP proteins. Current research on RAB3IP is being conducted in relation to vitelliform, macular dystrophy, pilocytic astrocytoma, anaplastic astrocytoma and astrocytoma. RAB3IP is linked to the vesicle docking, metaphase, exocytosis, mitosis, transport, synaptic transmission and cytokinesis pathways where it interacts with RAB3IL1, SSX2B, SSX2, RAB8A and RAB3A.

Alternate Names

FLJ14660, FLJ22548, MGC71495, RAB3A interacting protein (rabin3), rab-3A-interacting protein, Rab3A-interacting protein, RABIN3, rabin-3, SSX2 interacting protein, SSX2-interacting protein

Gene Symbol

RAB3IP

Additional RAB3IP Products

Product Documents for RAB3IP Antibody (OTI5F2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RAB3IP Antibody (OTI5F2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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