Rab4 Antibody (8I5K1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16307

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16307-100ul
NBP3-16307-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8I5K1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Rab4 Antibodies »

Product Summary for Rab4 Antibody (8I5K1)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Rab4 (P20338). MSQTAMSETYDFLFKFLVIGNAGTGKSCLLHQFIEKKFKDDSNHTIGVEFGSKIINVGGKYVKLQIWDTAGQERFRSVTRSYYRGAAGALLVYDITSRET

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rab4 Antibody (8I5K1)

Western Blot: Rab4 Antibody (8I5K1) [NBP3-16307]

Western Blot: Rab4 Antibody (8I5K1) [NBP3-16307]

Western Blot: Rab4 Antibody (8I5K1) [NBP3-16307] - Analysis of extracts of various cell lines, using Rab4 Rabbit mAb (NBP3-16307) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for Rab4 Antibody (8I5K1)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rab4

Rab4 is a small GTPase that regulates recycling of proteins, such as GPCRs, from the early endosomes to the cell surface. Overexpression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. Rab4 is also involved in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin. Association of Rab4 with transferrin receptor containing early endosomes is mediated through the geranylgeranyl groups at its carboxyl terminus. Rab4 can be used as a marker for early endosomes (see Daro et al. 1996 and Zuk et al. 1999 for more information). Membrane association is also cell cycle dependent, as phosphorylation at its C-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol.

Alternate Names

Oncogene RAB4, RAB4, member RAS oncogene family, RAB4A, member RAS oncogene family, RAB4HRES-1/RAB4, ras-related protein Rab-4A

Gene Symbol

RAB4A

Additional Rab4 Products

Product Documents for Rab4 Antibody (8I5K1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rab4 Antibody (8I5K1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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