RAB8A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93433

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB100-93433
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all RAB8A Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NQGVKITPDQQKR corresponding to C-Terminus according to NP_005361.2.

Epitope

C-NQGVKITPDQQKR

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RAB8A Antibody

Western Blot: RAB8A Antibody [NB100-93433]

Western Blot: RAB8A Antibody [NB100-93433]

Western Blot: RAB8A Antibody [NB100-93433] - (0.1ug/ml) staining of Human Brain (Cerebellum) 35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for RAB8A Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 25 kDa band observed in human brain (cerebellum), kidney and heart lysates (calculated MW of 23.7 kDa band according to NP_005361.2).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAB8A

The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. [provided by RefSeq]

Long Name

Ras-related protein Rab-8A

Alternate Names

MEL, Oncogene c-mel, RAB8

Entrez Gene IDs

4218 (Human)

Gene Symbol

RAB8A

UniProt

NP_005361.2 (Human)

Additional RAB8A Products

Product Documents for RAB8A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RAB8A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.