Rad1 Antibody (1G2)

Novus Biologicals, part of Bio-Techne | Catalog # H00005810-M01J

Novus Biologicals, part of Bio-Techne
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H00005810-M01J-0.1mg
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Sandwich ELISA, Sandwich ELISA Capture, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 1G2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Rad1 Antibodies »

Product Specifications

Immunogen

RAD1 (AAH06837, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQAGIFQEFKVQEESVTFRINLTVLL

Specificity

RAD1 homolog (S. pombe)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Scientific Data Images for Rad1 Antibody (1G2)

Western Blot: Rad1 Antibody (1G2) [H00005810-M01J]

Western Blot: Rad1 Antibody (1G2) [H00005810-M01J]

Western Blot: Rad1 Antibody (1G2) [H00005810-M01J] - Western blot analysis of Rad1 over-expressed 293 cell line, cotransfected with Rad1 Validated Chimera RNAi [H00005810-R01V] (Lane 2) or non-transfected control (Lane 1). Blot probed with Rad1 monoclonal antibody (M01), clone 1G2 [H00005810-M01]. GAPDH ( 3
Immunohistochemistry-Paraffin: Rad1 Antibody (1G2) [H00005810-M01J]

Immunohistochemistry-Paraffin: Rad1 Antibody (1G2) [H00005810-M01J]

Immunohistochemistry-Paraffin: Rad1 Antibody (1G2) [H00005810-M01J] - Immunoperoxidase of monoclonal antibody to Rad1 on formalin-fixed paraffin-embedded human lateral ventricle wall. [antibody concentration 3 ug/ml]
Western Blot: Rad1 Antibody (1G2) [H00005810-M01J]

Western Blot: Rad1 Antibody (1G2) [H00005810-M01J]

Western Blot: Rad1 Antibody (1G2) [H00005810-M01J] - Analysis of RAD1 expression in transfected 293T cell line by RAD1 monoclonal antibody (M01J), clone 1G2. Lane 1: RAD1 transfected lysate(32.43 KDa).Lane 2: Non-transfected lysate.
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Applications for Rad1 Antibody (1G2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

1X PBS pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rad1

This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

Alternate Names

cell cycle checkpoint protein Hrad1, checkpoint control protein HRAD1, DNA repair exonuclease rad1 homolog, DNA repair exonuclease REC1, EC 3.1.11.2, exonuclease homolog RAD1, HRAD1, RAD1 (S. pombe) homolog, RAD1 homolog (S. pombe), Rad1-like DNA damage checkpoint protein, REC1cell cycle checkpoint protein RAD1

Entrez Gene IDs

5810 (Human)

Gene Symbol

RAD1

OMIM

603153 (Human)

UniProt

AAH06837 (Human)

Additional Rad1 Products

Product Documents for Rad1 Antibody (1G2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rad1 Antibody (1G2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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