Rad1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13196

Novus Biologicals, part of Bio-Techne
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NBP2-13196
NBP2-13196-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Rad1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: GLREAFSELDMTSEVLQITMSPDKPYFRLSTFGNAGSSHLDYPKDSDLMEAFHCNQTQVNRYKISLLKPSTKALVLSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPESES

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rad1 Antibody

Western Blot: Rad1 Antibody [NBP2-13196]

Western Blot: Rad1 Antibody [NBP2-13196]

Western Blot: Rad1 Antibody [NBP2-13196] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: Rad1 Antibody [NBP2-13196]

Immunocytochemistry/ Immunofluorescence: Rad1 Antibody [NBP2-13196]

Immunocytochemistry/Immunofluorescence: Rad1 Antibody [NBP2-13196] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Rad1 Antibody [NBP2-13196]

Immunohistochemistry-Paraffin: Rad1 Antibody [NBP2-13196]

Immunohistochemistry-Paraffin: Rad1 Antibody [NBP2-13196] - Staining of human urinary bladder shows moderate nuclear positivity in urothelial cells.

Applications for Rad1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rad1

Human Rad1 is a component of a heterotrimeric PCNA-like complex that also contains the Rad9 and Hus1 proteins. This complex is believed to be involved in cellular responses to DNA damage, possibly by associating with Rad17 and several components of the PCNA-loading heteropentamer, replication factor C. Human Rad1 exhibits significant homology to Rad1 from S. pombe, and its expression in yeast rad1 mutants has been shown to partially restore radiation resistance and G2-checkpoint activity. It has also been shown to possess exonuclease activity.

Alternate Names

cell cycle checkpoint protein Hrad1, checkpoint control protein HRAD1, DNA repair exonuclease rad1 homolog, DNA repair exonuclease REC1, EC 3.1.11.2, exonuclease homolog RAD1, HRAD1, RAD1 (S. pombe) homolog, RAD1 homolog (S. pombe), Rad1-like DNA damage checkpoint protein, REC1cell cycle checkpoint protein RAD1

Gene Symbol

RAD1

Additional Rad1 Products

Product Documents for Rad1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rad1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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