Rad1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97717

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Rad1 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human Rad1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Rad1 Antibody

Immunocytochemistry/ Immunofluorescence: Rad1 Antibody [NBP2-97717]

Immunocytochemistry/ Immunofluorescence: Rad1 Antibody [NBP2-97717]

Immunocytochemistry/Immunofluorescence: Rad1 Antibody [NBP2-97717] - Immunofluorescence staining of Rad1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Rad1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

Applications for Rad1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rad1

Human Rad1 is a component of a heterotrimeric PCNA-like complex that also contains the Rad9 and Hus1 proteins. This complex is believed to be involved in cellular responses to DNA damage, possibly by associating with Rad17 and several components of the PCNA-loading heteropentamer, replication factor C. Human Rad1 exhibits significant homology to Rad1 from S. pombe, and its expression in yeast rad1 mutants has been shown to partially restore radiation resistance and G2-checkpoint activity. It has also been shown to possess exonuclease activity.

Alternate Names

cell cycle checkpoint protein Hrad1, checkpoint control protein HRAD1, DNA repair exonuclease rad1 homolog, DNA repair exonuclease REC1, EC 3.1.11.2, exonuclease homolog RAD1, HRAD1, RAD1 (S. pombe) homolog, RAD1 homolog (S. pombe), Rad1-like DNA damage checkpoint protein, REC1cell cycle checkpoint protein RAD1

Gene Symbol

RAD1

Additional Rad1 Products

Product Documents for Rad1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rad1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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