Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Rad17 Antibodies »

Product Specifications

Immunogen

A synthetic peptide, which represented a portion of human Rad17 (LocusLink ID 5884) around serine 645.

Modification

p Ser645

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rad17 [p Ser645] Antibody

Western Blot: Rad17 [p Ser645] Antibody [NB100-273]

Western Blot: Rad17 [p Ser645] Antibody [NB100-273]

Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells treated with 100 uM EPE for 2 hours (+) or mock treated (-) cells. Antibodies : Affinity purified rabbit anti-Rad17, Phospho (S645) antibody . Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).
Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273]

Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273]

Immunohistochemistry: Rad17 [p Ser645] Antibody [NB100-273] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: Rad17 [p Ser645] Antibody [NB100-273]

Western Blot: Rad17 [p Ser645] Antibody [NB100-273]

Western Blot: Rad17 [p Ser645] Antibody [NB100-273] - Whole cell lysate (50 ug) from HeLa cells before (-) or after (+) treatment with 10 Gy ionizing radiation. Antibody used at 1 ug/ml.
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Applications for Rad17 [p Ser645] Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Western Blot

1:500-1:5000
Application Notes
IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Rad17

The RAD17 gene product of Schizosaccharomyces pombe is involved in the DNA damage checkpoint signal transduction pathway and has a key role in cell cycle checkpoint control.

Alternate Names

CCYC, cell cycle checkpoint protein (RAD17), cell cycle checkpoint protein RAD17, FLJ41520, hRad17, R24L, RAD1 (S. pombe) homolog, RAD1 homolog, RAD17 homolog (S. pombe), Rad17-like protein, RAD17Sp, Rad24, RF-C activator 1 homolog, RF-C/activator 1 homolog

Entrez Gene IDs

5884 (Human)

Gene Symbol

RAD17

Additional Rad17 Products

Product Documents for Rad17 [p Ser645] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rad17 [p Ser645] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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