Rad17 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75345

Novus Biologicals, part of Bio-Techne
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NBP2-75345
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Rad17 Antibodies »

Product Summary for Rad17 Antibody

Immunogen

Between 564 and 614

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rad17 Antibody

Western Blot: Rad17 Antibody [Unconjugated] [NBP2-75345] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Detection by chemiluminescence.
Immunoprecipitation: Rad17 Antibody [Unconjugated] [NBP2-75345] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. For blotting immunoprecipitated Rad17, antibody was used at 1:100. Detection by chemiluminescence with an exposure time of 30 seconds.

Applications for Rad17 Antibody

Application
Recommended Usage

Immunoprecipitation

40 ul/mg lysate

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

TBS, 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: Rad17

Rad17 is a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O75943].

Alternate Names

CCYC, cell cycle checkpoint protein (RAD17), cell cycle checkpoint protein RAD17, FLJ41520, hRad17, R24L, RAD1 (S. pombe) homolog, RAD1 homolog, RAD17 homolog (S. pombe), Rad17-like protein, RAD17Sp, Rad24, RF-C activator 1 homolog, RF-C/activator 1 homolog

Gene Symbol

RAD17

Additional Rad17 Products

Product Documents for Rad17 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rad17 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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