RAD18 Antibody (79B1048.1)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56523

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 79B1048.1

Concentration

0.5 mg/ml

View all RAD18 Antibodies »

Product Specifications

Immunogen

The antibody was raised against a synthetic peptide corresponding to the amino acids 402-414 of human Rad18.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for RAD18 Antibody (79B1048.1)

Western Blot: RAD18 Antibody (79B1048.1) [NB100-56523]

Western Blot: RAD18 Antibody (79B1048.1) [NB100-56523]

Western Blot: RAD18 Antibody (79B1048.1) [NB100-56523] - The anti-Rad18 mAb was diluted at 2 ug/ml and tested against 20 ug of HL-60 cell lysate.

Applications for RAD18 Antibody (79B1048.1)

Application
Recommended Usage

Western Blot

2-4 ug/ml
Application Notes
In HL60, a 54 kDa band should be detected.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.2% gelatin

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAD18

Human Rad18 (hRad18) is homologue of the S. pombe Rad18 (3,4). In yeast, Rad6 and Rad18 play a crucial role in post-replication repair functions in gap-filling of a daughter strand on replication of damaged DNA (2). Furthermore, Rad6-Rad18 interaction is thought to be critical at an early step during lesion bypass in yeast. In vivo, Rad18 protein forms a tight complex with Rad6 through a conserved ring-finger motif (3,4). It has been suggested that Rad18-Rad6 complex binds to the damaged sites by the single-stranded DNA binding property of Rad 18. This is followed by degradation of the stalled components of DNA replication by ubiquitin-conjugating enzyme activity of Rad6 (1). The human Rad18 maps on chromosome 3p24-25, the region which is often deleted in lung, breast, ovary, and testis cancers. The human RAD18 gene codes for a protein of 495 amino acids with a predicted molecular weight of 56 kDa. The human Rad18 is ubiquitously expressed in various human tissues.

Alternate Names

EC 6.3.2.-, hHR18, hRAD18, postreplication repair protein hRAD18p, Postreplication repair protein RAD18, RAD18 homolog (S. cerevisiae), RAD18, S. cerevisiae, homolog, RING finger protein 73, RNF73E3 ubiquitin-protein ligase RAD18

Entrez Gene IDs

56852 (Human)

Gene Symbol

RAD18

Additional RAD18 Products

Product Documents for RAD18 Antibody (79B1048.1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RAD18 Antibody (79B1048.1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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