Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Rad21 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 575 and the C-terminus (residue 631) human Rad21 homolog using the numbering given in entry NP_006256.1 (GeneID 5885).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rad21 Antibody

Rad21 Antibody [NB100-203] - Detection of human and mouse Rad21 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Jurkat, HEK293T, RKO, GaMG, MCF-7, A-549, U2OS, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Rad21 antibody NB100-203 used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rad21 Antibody [NB100-203] - Detection of human Rad21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Rad21 antibody NB100-203 (Lot NB100-203-8) used for IP at 6 ug per reaction. Rad21 was also immunoprecipitated by a previous lot of this antibody (lot NB100-203-6). For blotting immunoprecipitated Rad21, rabbit anti-Rad21 recombinant monoclonal antibody NB100-203 was used at 1:1000. Chemiluminescence with an exposure time of 3 minutes.Previous

Applications for Rad21 Antibody

Application
Recommended Usage

Immunoprecipitation

2- 10 ug/mg of lysate

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Rad21

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

Alternate Names

double-strand-break repair protein rad21 homolog, hHR21FLJ25655, HR21, KIAA0078RAD21 (S. pombe) homolog, MCD1, Nuclear matrix protein 1, NXP-1, NXP1HRAD21, protein involved in DNA double-strand break repair, RAD21 homolog (S. pombe), SCC1 homolog, SCC1FLJ40596

Gene Symbol

RAD21

Additional Rad21 Products

Product Documents for Rad21 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rad21 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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