Rad23 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49977

Novus Biologicals, part of Bio-Techne
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NBP1-49977
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Rad23 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 200 and 250 of human RAD23 Homolog A using the numbering given in entry NP_005044.1 (GeneID 5886).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rad23 Antibody

Western Blot: Rad23 Antibody [NBP1-49977]

Western Blot: Rad23 Antibody [NBP1-49977]

Western Blot: Rad23 Antibody [NBP1-49977] - Detection of Human and Mouse RAD23A by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RAD23A antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. For blotting immunoprecipitated RAD23A was used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Immunohistochemistry: Rad23 Antibody [NBP1-49977]

Immunohistochemistry: Rad23 Antibody [NBP1-49977]

Immunohistochemistry: Rad23 Antibody [NBP1-49977] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-RAD23A used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Applications for Rad23 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Rad23

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Alternate Names

hHR23

Gene Symbol

RAD23A

Additional Rad23 Products

Product Documents for Rad23 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rad23 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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