Rad51 Antibody (13E4)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-499

Novus Biologicals, part of Bio-Techne
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NB100-499
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 13E4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Rad51 Antibodies »

Product Specifications

Immunogen

Recombinant human Rad51 protein.

Localization

Nuclear

Specificity

This is specific for Rad51.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rad51 Antibody (13E4)

Western Blot: Rad51 Antibody (13E4) [NB100-499]

Western Blot: Rad51 Antibody (13E4) [NB100-499]

Western Blot: Rad51 Antibody (13E4) [NB100-499] - Detection of Rad51 in HeLa whole cell extract.
Western Blot: Rad51 Antibody (13E4) [NB100-499]

Western Blot: Rad51 Antibody (13E4) [NB100-499]

Western Blot: Rad51 Antibody (13E4) [NB100-499] - Sample (50ug of whole cell lysate) A: mouse testis 10% SDS PAGE diluted at 1:500

Applications for Rad51 Antibody (13E4)

Application
Recommended Usage

Western Blot

1:500-1:1000
Application Notes
Use in ICC/IF reported in scientific literature (PMID:35152263) IHC usage reported in (PMID: 23480638). .
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rad51

Rad51, a 37 kDa protein, is the human homologue of E. coli RecA protein and a member of the RAD52 epistasis group in S. cerevisiae. In recent studies it has been reported that BRCA1 interacts with Rad51 and disease-causing mutations have been found in the BRCA1 region necessary for BRCA1/Rad51 interaction, implying that this interaction is important for tumor suppression. BRCA2 and Rad51 have also been shown to interact by direct binding of the BRC repeats located within exon 11 of BRCA2. When these repeats are deleted, the interaction is lost and cells become hypersensitive to the DNA damage caused by methyl methanesulfonate (MMS).

Alternate Names

BRCC5, hRAD51, HsRAD51, HsT16930, RAD51 (S. cerevisiae) homolog (E coli RecA homolog), RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51 homolog A, RAD51ABRCA1/BRCA2-containing complex, subunit 5, RAD51L3, RecA, E. coli, homolog of, RECADNA repair protein RAD51 homolog 1, RecA-like protein, recombination protein A

Entrez Gene IDs

5888 (Human)

Gene Symbol

RAD51

Additional Rad51 Products

Product Documents for Rad51 Antibody (13E4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rad51 Antibody (13E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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