Rad51C Antibody (3F3-5C6)
Novus Biologicals, part of Bio-Techne | Catalog # H00005889-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3F3-5C6
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
RAD51C (AAH00667.1, 1 a.a. ~ 134 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRGKTFRFEMQRDLVSFPLSPAVRVKLVSAGFQTAEELLEVKPSELSKEVGISKAEALETLQIIRRECLTNKPRYAGTSESHKKCTALELLEQEHTQGFIITFCSALDDILGGGVPLMKTTEICGAPGVGKTQL
Localization
Nuclear
Specificity
RAD51C - RAD51 homolog C (S. cerevisiae)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Rad51C Antibody (3F3-5C6)
Western Blot: Rad51C Antibody (3F3-5C6) [H00005889-M01]
Western Blot: Rad51C Antibody (3F3-5C6) [H00005889-M01] - Analysis of RAD51C expression in transfected 293T cell line by RAD51C monoclonal antibody (M01), clone 3F3-5C6.Lane 1: RAD51C transfected lysate(42.2 KDa).Lane 2: Non-transfected lysate.Immunocytochemistry/ Immunofluorescence: Rad51C Antibody (3F3-5C6) [H00005889-M01]
Immunocytochemistry/Immunofluorescence: Rad51C Antibody (3F3-5C6) [H00005889-M01] - Analysis of monoclonal antibody to RAD51C on HeLa cell. Antibody concentration 10 ug/ml.Western Blot: Rad51C Antibody (3F3-5C6) [H00005889-M01]
Western Blot: Rad51C Antibody (3F3-5C6) [H00005889-M01] - Analysis of RAD51C over-expressed 293 cell line, cotransfected with RAD51C Validated Chimera RNAi ( Cat # H00005889-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD51C monoclonal antibody (M01), clone 3F3-5C6 (Cat # H00005889-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Applications for Rad51C Antibody (3F3-5C6)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Rad51C
Alternate Names
BROVCA3, DNA repair protein RAD51 homolog 3, FANCO, MGC104277, R51H3, RAD51 homolog C, RAD51 homolog C (S. cerevisiae), RAD51L2RAD51 (S. cerevisiae) homolog C, RAD51-like protein 2, yeast RAD51 homolog 3
Entrez Gene IDs
5889 (Human)
Gene Symbol
RAD51C
OMIM
602774 (Human)
Additional Rad51C Products
Product Documents for Rad51C Antibody (3F3-5C6)
Product Specific Notices for Rad51C Antibody (3F3-5C6)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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