Rad51D Antibody (5B3/6)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-178

Novus Biologicals, part of Bio-Techne
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NB100-178
NB100-178SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse (Negative)

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5B3/6

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Rad51D Antibodies »

Product Specifications

Immunogen

His-tagged human Rad51D, overexpressed in E. coli. [UniProt# O75771]

Reactivity Notes

This antibody does not work on mouse.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rad51D Antibody (5B3/6)

Western Blot: Rad51D Antibody (5B3/6) [NB100-178]

Western Blot: Rad51D Antibody (5B3/6) [NB100-178]

Western Blot: Rad51D Antibody (5B3/6) [NB100-178] - Rad51D detected in HEK293 lysates using a 1:1,000 dilution of NB 100-178 (purified). Photo courtesy of B.T. Bennett & K. Knight of University of Massachusetts Medical School
Immunocytochemistry/ Immunofluorescence: Rad51D Antibody (5B3/6) [NB100-178]

Immunocytochemistry/ Immunofluorescence: Rad51D Antibody (5B3/6) [NB100-178]

Immunocytochemistry/Immunofluorescence: Rad51D Antibody (5B3/6) [NB100-178] - RAD51D antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).

Applications for Rad51D Antibody (5B3/6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Western Blot

1:1000
Application Notes
This Rad51D (5B3/6) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~40 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Rad51D

Five human RAD51 homologues have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homologues interacts with one or more of the others, with all of the proteins involved in one complex or multiple smaller complexes. RAD51D interacts with XRCC2 and RAD51C (1). RAD51D's expression pattern and sequence similarity to other RAD51 family members most likely make it part of a complex of proteins required for DNA repair and meiotic recombination.

Alternate Names

DNA repair protein RAD51 homolog 4, HsTRAD, R51H3Trad, RAD51 homolog D, RAD51DRAD51 (S. cerevisiae)-like 3, RAD51-like 3 (S. cerevisiae), RAD51-like protein 3, recombination repair protein, TRAD

Gene Symbol

RAD51D

Additional Rad51D Products

Product Documents for Rad51D Antibody (5B3/6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rad51D Antibody (5B3/6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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