Radixin Antibody (1D9)

Novus Biologicals, part of Bio-Techne | Catalog # H00005962-M06

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Cited:

Avian - Chicken

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1D9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Radixin Antibodies »

Product Specifications

Immunogen

RDX (AAH47109, 1 a.a. ~ 583 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTVGLREVWFFGLQYVDSKGYSTWLKLNKKVTQQDVKKENPLQFKFRAKFFPEDVSEELIQEITQRLFFLQVKEAILNDEIYCPPETAVLLASYAVQAKYGDYNKEIHKPGYLANDRLLPQRVLEQHKLTKEQWEERIQNWHEEHRGMLREDSMMEYLKIAQDLEMYGVNYFEIKNKKGTELWLGVDALGLNIYEHDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILALCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERAQLENEKEKREIAEKEKERIEREKEELMERLKQIEEQTIKAQKELEEQTRKALELDQERKRAKEEAERLEKERRAAEEAKSAIAKQAADQMKNQEQLAAELAEFTAKIALLEEAKKKKEEEATEWQHKAFAAQEDLEKTKEELKTVMSAPPPPPPPPVIPPTENEHDEHDENNAEASAELSNEGVMNHRSEEERVTETQKNERVKKQLQALSSELAQARDETKKTQNDVLHAENVKAGRDKYKTLRQIRQGNTKQRIDEFEAM

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 22457506).

Specificity

RDX - radixin

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Radixin Antibody (1D9)

Western Blot: Radixin Antibody (1D9) [H00005962-M06]

Western Blot: Radixin Antibody (1D9) [H00005962-M06]

Western Blot: Radixin Antibody (1D9) [H00005962-M06] - Analysis of RDX expression in HeLa (Cat # L013V1).
Immunocytochemistry/ Immunofluorescence: Radixin Antibody (1D9) [H00005962-M06]

Immunocytochemistry/ Immunofluorescence: Radixin Antibody (1D9) [H00005962-M06]

Immunocytochemistry/Immunofluorescence: Radixin Antibody (1D9) [H00005962-M06] - Analysis of monoclonal antibody to RDX on HeLa cell. Antibody concentration 30 ug/ml
Immunohistochemistry: Radixin Antibody (1D9) [H00005962-M06]

Immunohistochemistry: Radixin Antibody (1D9) [H00005962-M06]

Immunohistochemistry: Radixin Antibody (1D9) [H00005962-M06] - Decreased phosphorylation of radixin in the RMS after DX52-1 treatment. Merged image of radixin and phosphorylated rzrin/radixin/moesin (pERM) immunoreactivity in a coronal section of the control RMS. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fncel.2013.00161/abstract) licensed under a CC-BY license.
View 1 more images

Applications for Radixin Antibody (1D9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Radixin

Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses.

Alternate Names

deafness, autosomal recessive 24, DFNB24, radixin

Entrez Gene IDs

5962 (Human)

Gene Symbol

RDX

OMIM

179410 (Human)

UniProt

AAH47109 (Human)

Additional Radixin Products

Product Documents for Radixin Antibody (1D9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Radixin Antibody (1D9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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