Radixin Antibody (1E12)

Immunocytochemistry/ Immunofluorescence: Radixin Antibody (1E12) [H00005962-M01] -

Immunocytochemistry/ Immunofluorescence: Radixin Antibody (1E12) [H00005962-M01] - Localization studies of ERM proteins, Ebp50 & ErbB2 in SKBR3 cells & protein proximity analysis(A) Confocal microscopy of ERM proteins & Ebp50. Untreated cells were fixed, permeabilized & stained by specific antibodies for endogenous levels of ezrin, radixin & Ebp50 (max. projection). In SKBR3 cells ezrin & radixin strongly colocalizes with Ebp50 at the plasma membrane. (B) Confocal microscopy (single plane section) & (C) 3D-SIM (max. projections) of ErbB2 & Ebp50 localization. In untreated control conditions, ErbB2 shows a strong colocalization with Ebp50 in lamellipodia-like structures at the plasma membrane. (D-F) Analysis of protein proximity by PLA experiments. SKBR3 cells were transfected either with non-targeting control siRNA or ezrin/radixin specific siRNA. Cells were fixed 72 h after transfection & PLA experiments were performed. The depletion of ezrin or radixin strongly reduces the interaction of ErbB2 with Ebp50 (D), Hsp90 (E) & ErbB3 (F). All results are shown as mean +/− SEM (*P < 0.05; ***P < 0.001). Scale bars: 10 μm (A, B), 8 μm (C). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27029001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Radixin Antibody (1E12) [H00005962-M01] -

Western Blot: Radixin Antibody (1E12) [H00005962-M01] - ERM expression & localization in SKBR3 breast cancer cells(A) Western blot analysis of ERM levels in SKBR3, HeLa & PC3 cells. SKBR3 breast cancer cells do not express moesin. (B) Colocalization of ezrin/radixin & ErbB2 in SKBR3 cells. 3D-SIM of fixed cells, stained for endogenous ERM & ErbB2, shows a high degree of colocalization between the ezrin/radixin & ErbB2 at the plasma membrane (left panel: max. projection; middle: single plane section; right: single channels of insert). Scale bars: 10 μm. (C) Analysis of protein association in SKBR3 cells by proximity ligation assay (PLA). 2 h treatment with 3 μM GA leads to decreased association of ezrin/ErbB2 & radixin/ErbB2. Data is represented as mean +/− SEM (***P < 0.001). (D) Corresponding single plan section of a representative PLA experiment. Fluorescence & DIC pictures of control cells (upper panel) & cell treated for 2 h with geldanamycin (lower panel) are shown. Scale bars: 10 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27029001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Radixin Antibody (1E12) [H00005962-M01] -

Immunocytochemistry/ Immunofluorescence: Radixin Antibody (1E12) [H00005962-M01] - ERM expression & localization in SKBR3 breast cancer cells(A) Western blot analysis of ERM levels in SKBR3, HeLa & PC3 cells. SKBR3 breast cancer cells do not express moesin. (B) Colocalization of ezrin/radixin & ErbB2 in SKBR3 cells. 3D-SIM of fixed cells, stained for endogenous ERM & ErbB2, shows a high degree of colocalization between the ezrin/radixin & ErbB2 at the plasma membrane (left panel: max. projection; middle: single plane section; right: single channels of insert). Scale bars: 10 μm. (C) Analysis of protein association in SKBR3 cells by proximity ligation assay (PLA). 2 h treatment with 3 μM GA leads to decreased association of ezrin/ErbB2 & radixin/ErbB2. Data is represented as mean +/− SEM (***P < 0.001). (D) Corresponding single plan section of a representative PLA experiment. Fluorescence & DIC pictures of control cells (upper panel) & cell treated for 2 h with geldanamycin (lower panel) are shown. Scale bars: 10 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27029001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.