Radixin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31553

Novus Biologicals, part of Bio-Techne
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NBP1-31553
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Radixin Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Radixin. The exact sequence is proprietary.

Localization

Cell membrane, Peripheral membrane protein, Cytoplasmic side, Cytoplasm, cytoskeleton

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Radixin Antibody

Western Blot: Radixin Antibody [NBP1-31553]

Western Blot: Radixin Antibody [NBP1-31553]

Western Blot: Radixin Antibody [NBP1-31553] - Wild-type (WT) and Radixin knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Radixin antibody (NBP1-31553) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Radixin Antibody [NBP1-31553]

Immunocytochemistry/ Immunofluorescence: Radixin Antibody [NBP1-31553]

Immunocytochemistry/Immunofluorescence: Radixin Antibody [NBP1-31553] - Analysis of HeLa, using NBP1-31553 at 1:200 dilution.
Immunohistochemistry-Paraffin: Radixin Antibody [NBP1-31553]

Immunohistochemistry-Paraffin: Radixin Antibody [NBP1-31553]

Immunohistochemistry-Paraffin: Radixin Antibody [NBP1-31553] - RT2 xenograft, using Radixin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Applications for Radixin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
IP-Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Radixin

Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. Recent cloning of the murine and porcine radixin cDNAs revealed a protein highly homologous to ezrin and moesin (1). The ezrin-radixin-moesin (ERM) family of proteins crosslink actin filaments and integral membrane proteins. Radixin (encoded by Rdx) is the dominant ERM protein in the liver of wildtype mice and is concentrated at bile canalicular membranes (BCMs). Findings indicate that radixin is required for secretion of conjugated bilirubin through its support of Mrp2 localization at BCMs (2). Proteins of the ezrin-radixin-moesin family are ubiquitous constituents of the submembrane cortex, especially in epithelial cells. Radixin is thus a prominent constituent of stereocilia, where it may participate in anchoring the pointed ends of actin filaments to the membrane (3).

Alternate Names

deafness, autosomal recessive 24, DFNB24, radixin

Gene Symbol

RDX

Additional Radixin Products

Product Documents for Radixin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Radixin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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