RAE1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1894

Novus Biologicals, part of Bio-Techne
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NB100-1894
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all RAE1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NAAEELKPRNKK corresponding to C-Terminus according to NP_003601.1, NP_001015885.1.

Specificity

NP_003601and NP_001015885 represent identical proteins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RAE1 Antibody

Western Blot: RAE1 Antibody [NB100-1894]

Western Blot: RAE1 Antibody [NB100-1894]

Western Blot: RAE1 Antibody [NB100-1894] - Staining of HepG2 (A), HeLa (B), Jurkat (C) and MCF7 (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: RAE1 Antibody [NB100-1894]

Immunohistochemistry-Paraffin: RAE1 Antibody [NB100-1894]

Immunohistochemistry-Paraffin: RAE1 Antibody [NB100-1894] - Staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RAE1 Antibody [NB100-1894]

Immunohistochemistry-Paraffin: RAE1 Antibody [NB100-1894]

Immunohistochemistry-Paraffin: RAE1 Antibody [NB100-1894] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for RAE1 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 6 ug/ml

Immunohistochemistry-Paraffin

3 - 6 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 40 kDa band observed in lysates HepG2, HeLa, Jurkat and MCF7 (calculated MW of 41.0 kDa band according to NP_003601.1) . IHC: Human brain cortex shows staining of neuronal cell bodies.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAE1

Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Alternate Names

dJ481F12.3, dJ800J21.1, FLJ30608, homolog of yeast Rae1 (Bharathi) mRNA-associated protein of 41 kDa (Kraemer), MGC117333, MGC126076, MGC126077, MIG14, migration-inducing gene 14, Mnrp41, mRNA export factor, mRNA export protein, mRNA-associated protein mrnp 41, mRNA-binding protein, 41-kD, MRNP41, RAE1 (RNA export 1, S.pombe) homolog, Rae1 protein homolog, RAE1 RNA export 1 homolog (S. pombe)

Entrez Gene IDs

8480 (Human); 66679 (Mouse); 362281 (Rat)

Gene Symbol

RAE1

UniProt

NP_003601.1 (Human)

Additional RAE1 Products

Product Documents for RAE1 Antibody

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Certificate of Analysis

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Product Specific Notices for RAE1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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