RAG2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43666
Key Product Details
Species Reactivity
Validated:
Human, Rat
Predicted:
Bovine (91%), Porcine (90%), Rabbit (91%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for RAG2 Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human RAG2. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RAG2 Antibody
Western Blot: RAG2 Antibody [NBP2-43666]
Western Blot: RAG2 Antibody [NBP2-43666] - Analysis of A. 50 ug rat thymus lysate/extract 7.5 % SDS-PAGE RAG2 antibody dilution: 1:1000.Immunocytochemistry/ Immunofluorescence: RAG2 Antibody [NBP2-43666]
Immunocytochemistry/Immunofluorescence: RAG2 Antibody [NBP2-43666] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAG2 protein stained by RAG2 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10um.Immunohistochemistry-Paraffin: RAG2 Antibody [NBP2-43666]
Immunohistochemistry-Paraffin: RAG2 Antibody [NBP2-43666] - DLD1 Xenograft, using RAG2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Applications for RAG2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7)
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RAG2
Alternate Names
RAG-2, recombination activating gene 2, V(D)J recombination-activating protein 2
Gene Symbol
RAG2
Additional RAG2 Products
Product Documents for RAG2 Antibody
Product Specific Notices for RAG2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.