Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all RAI1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human retinoic acid induced 1 using the numbering given in entry NP_109590.3 (GeneID 10743).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAI1 Antibody

Immunohistochemistry-Paraffin: RAI1 Antibody [NBP1-28745]

Immunohistochemistry-Paraffin: RAI1 Antibody [NBP1-28745]

Immunohistochemistry-Paraffin: RAI1 Antibody [NBP1-28745] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RAI1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: RAI1 Antibody [NBP1-28745]

Immunoprecipitation: RAI1 Antibody [NBP1-28745]

Immunoprecipitation: RAI1 Antibody [NBP1-28745] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RAI1 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. RAI1 was also immunoprecipitated by rabbit anti-RAI1 antibody BL6549, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for RAI1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RAI1

Defects in Retinoic acid-induced protein 1 (RAI1) are a cause of Smith-Magenis syndrome (SMS) . SMS is characterized by congenital mental retardation associated with development and growth delays. The RAI1 has also been associated with schizophrenia.

Alternate Names

KIAA1820DKFZP434A139, MGC12824, retinoic acid induced 1, retinoic acid-induced protein 1, SMCR, Smith-Magenis syndrome chromosome region, SMSDKFZp434A139

Entrez Gene IDs

10743 (Human)

Gene Symbol

RAI1

UniProt

NP_109590.3 (Human)

Additional RAI1 Products

Product Documents for RAI1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RAI1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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