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RAIDD/CRADD Antibody (1F8)

Novus Biologicals, part of Bio-Techne | Catalog # H00008738-M01

Novus Biologicals, part of Bio-Techne
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H00008738-M01

Key Product Details

Species Reactivity

Human, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1F8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

CRADD (AAH37905, 1 a.a. ~ 199 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEARDKQVLRSLRLELGAEVLVEGLVLQYVYEEGILTENHIQEINAQTTGLRKTMLLLDILPSRGPKAFDTFLDSLQEFPWVREKLKKAREEAMTDLPAGDRLTGIPSHILNSSPSDRQINQLAQRLGPEWEPMVLSLGLSQTDIYRCKANHPHNVQSQVVEAFIRWRQRFGKQATFQSLHNGLRAVEVDPSLLLHMLE

Specificity

CRADD - CASP2 and RIPK1 domain containing adaptor with death domain

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RAIDD/CRADD Antibody (1F8)

Western Blot: RAIDD/CRADD Antibody (1F8) [H00008738-M01]

Western Blot: RAIDD/CRADD Antibody (1F8) [H00008738-M01]

Western Blot: RAIDD/CRADD Antibody (1F8) [H00008738-M01] - CRADD monoclonal antibody (M01), clone 1F8 Analysis of CRADD expression in K-562.
Immunocytochemistry/ Immunofluorescence: RAIDD/CRADD Antibody (1F8) [H00008738-M01]

Immunocytochemistry/ Immunofluorescence: RAIDD/CRADD Antibody (1F8) [H00008738-M01]

Immunocytochemistry/Immunofluorescence: RAIDD/CRADD Antibody (1F8) [H00008738-M01] - Analysis of monoclonal antibody to CRADD on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: RAIDD/CRADD Antibody (1F8) [H00008738-M01]

Western Blot: RAIDD/CRADD Antibody (1F8) [H00008738-M01]

Western Blot: RAIDD/CRADD Antibody (1F8) [H00008738-M01] - Analysis of CRADD expression in transfected 293T cell line by CRADD monoclonal antibody (M01), clone 1F8.Lane 1: CRADD transfected lysate(23 KDa).Lane 2: Non-transfected lysate.

Applications for RAIDD/CRADD Antibody (1F8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CRADD

The protein encoded by this gene is a death domain (CARD/DD)-containing protein and has been shown to induce cell apoptosis. Through its CARD domain, this protein interacts with, and thus recruits, caspase 2/ICH1 to the cell death signal transduction complex that includes tumor necrosis factor receptor 1 (TNFR1A), RIPK1/RIP kinase, and numbers of other CARD domain-containing proteins.

Long Name

CASP2 and RIPK1 Domain Containing Adaptor With Death Domain

Alternate Names

RAIDD

Entrez Gene IDs

8738 (Human)

Gene Symbol

CRADD

OMIM

603454 (Human)

Additional CRADD Products

Product Documents for RAIDD/CRADD Antibody (1F8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RAIDD/CRADD Antibody (1F8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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